Why freeze-dried PCR mixes?

Freeze-dried/lyophilised PCR mixes are stable at ambient temperatures and allow point-of-care use without any refrigerating infrastructure (Fig. 1). Simply fill up vials with template DNA and run PCR as usual.

Dry PCR mixes are stable at 30 °C for > 1 year

Fig. 1: Dry PCR mixes remain fully functional even after 12 months heat exposure (30 °C, green circle) while liquid mixes significantly lose activity even when stored in the fridge (4 °C, red circle). (Assay: Amplification of a 4 kb fragment of lambda DNA in a template dilution series down to 10 pg).

Benefits:

• Ready-to-use: No thawing of PCR components
• Stability: dramatically enhance shelf life (Fig. 1)
• Activity: No loss caused by freeze-thaw cycles
• Avoid contamination: receive dry mix in aliquots
• Customized: Available in all common PCR plastic ware