The Ehrlichia Antibody 2.0 Rapid Test is a test cassette to diagnose the presence of Ehrlichia antibodies in dog's serum specimen. The Ehrlichia canis antibody and Ehrlichia ewingii antibody could be detected out by this rapid test. Assay Time: 5-10 minutes Specimen: Serum, plasma.
PRINCIPLE
The Ehrlichia Antibody 2.0 Rapid Test is based on sandwich lateral flow immunochromatographic assay.
REAGENTS AND MATERIALS
Test devices
Disposable capillary droppers
Assay buffers
Products Manual
STORAGE AND STABILITY
The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (24 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.
SPECIMEN PREPARATION AND STORAGE
Specimen should be obtained and treated as below. Serum or plasma: collect the whole blood from the patient dog, centrifuge it to get the plasma, or place the whole blood into a tube which contains anticoagulants to get serum.
2. All specimen should be tested immediately. If not for testing right now, they should be stored at 2-8ºC.
TEST PROCEDURE
Allow all materials, including specimen and test device, recover to 15-25ºC before running the assay.
Take out the test card from the foil pouch and place it horizontally.
Collect 20μL of the prepared specimen into a vial of assay buffer and mix well. Then drop 3 drops (approx. 120μL) of the diluted sample into the sample hole "S" of the test card.
Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
INTERPRETATION OF RESULTS
Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
Negative (-): Only clear C line appear. No T line.
Invalid: No colored line appears in C zone. No matter if T line appears.
PRECAUTIONS
All reagents must be at room temperature before running the assay.
Do not remove test cassette from its pouch until immediately before use.
Do not use the test beyond its expiration date.
The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.
LIMITATION
The Ehrlichia Antibody 2.0 Rapid Test is for in vitro veterinary diagnosis use only. All result should be considered with other clinical information available with veterinarian. It is suggested to apply a further confirmative method such as RT-PCR or microscopy when positive result was observed.
J&G Biotech Ltd (Reg. No.: 08419172) 326 Cleveland Road, London, England E18 2AN, UK
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