2× Universal Blue Sybr Green Qpcr Master Mix with Udg

The 2×Universal Blue SYBR Green qPCR Master Mix (with UDG) is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye-based indicators for accurate reaction set up. Coupled with user-supplied primer sets and template, the Master Mix is designed to amplifiy targets for accurate gene expression analysis.
it contains Taq DNA polymerase, which is tightly controlled through an antibody-mediated hot-start mechanism, helping prevent undesirable early activity of the polymerase at low temperatures that can lead to nonspecific amplification, and formulated with UNG/dUTP to prevent contamination of carryover PCR products. Meanwhile,it contains an inert blue dye that does not interfere with real-time PCR but provides increased visibility in the tube or well. A separate sample buffer is provided that contains an inert yellow dye. The optional use of this yellow sample buffer results in a reaction mixture that changes from blue to green upon addition of the sample, helping one avoid pipetting mistakes. The yellow sample buffer aids in reaction setup, but is not required to obtain superior results with the Master Mix.Those two blue and yellow dyes do not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection.

1. If pipetting tracking is not required, a dilute template with the 40×Yellow Template Dilution Buffer can be omitted.
2. Please Mix the reaction mix gently by inversion, then centrifuge briefly before aliquoting to the reaction plate to avoid bubbles.
3. Reagents should be placed on ice when preparing reaction mixes..
4. Strong light should be avoided when preparing PCR reaction mixes due to fluorescent dye SYBR Green.
5. New disposable tips should be used for preparation of reaction mixes to avoid cross-contamination
6. Avoid freeze-thawing cycles of the Master Mix, and try to use it up within a month after thawing.