Catalog No. | BD0042 |
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Product Name | Bioepitope® protein G Reagent |
Applications | Protein G is suitable for immunoprecipitation of mouse IgG1,IgG2a, IgG2b andIgG3, rat IgG1, IgG2a, IgG2b and IgG2c, rabbit and goat polyclonal Abs, andhuman IgG1, IgG2, IgG3 and IgG4 |
Alternative Name | |
Classification | IP Related Reagents |
Format | Liquid |
Contents | PBS,5mg Protein G |
Storage & Shelf Life | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. |
Procedure | Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. |
Research use | For research use only, not for use in diagnostic procedures. |
Expiration Date | One year from date of reconstitution. |
Background
MW: 58 kD (SDS-PAGE) Isoelectric point: 4.45 The pH range: pH 2.0-11.0 Purity: 95%