This product is a non-carcinogenic substitute for benzidine 1 (Ames test negative), suitable as a peroxidase substrate for ELISA. The substrate yields a soluble light blue final product that can be read spectrophotometrically at 370 or 6Chemicalbook 20-650nm. The TMB reaction can be stopped (turned yellow) with 2MH2SO4 and read at 450nm. A sensitive and specific reagent for detecting blood, measuring hemoglobin, and measuring peroxidase.
product name | Tetramethylbenzidine |
MF | C16H20N2 |
CAS | 54827-17-7 |
synonym | 1'-biphenyl)-4,4'-diamine,3,3',5,5'-tetramethyl-(;1'-biphenyl)-4,4-diamine,3,3',5,5'-tetramethyl-(;1'-Chemicalbookbiphenyl]-4,4'-diamine,3,3',5,5'-tetramethyl-[;TMB-E;TMBEASY;TMB/H;TMB-B;TMBBLUEONE-STEPSUBSTRATESYSTEM |