Hipure Ffpe Nucleic Acid Kit Based on Silica Gel Column Purification Technology

Min.Order: 1,000

Product origin: Guangzhou, Guangdong, China

Infringement complaint:

Complaint

US$ 2.48

Description

Product Name HiPure Tissue DNA Mini Kit
Cat. No. & Specifications D312602,50Preps/kit
Introduction
HiPure FFPE DNA Kit supplies a simple and rapid DNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, Southern Blot and viral DNA detection, ect.

Main Composition

Product	D3126-02
Purification times	50 Preps
HiPure DNA Mini Columns I	50
2ml Collection Tubes	50
Buffer DPS (Deparaffinization Buffer)	30 ml
Buffer ATL	15 ml
Buffer AL	15 ml
Buffer GW1*	13 ml
Buffer GW2*	20 ml
Proteinase K	22 mg
Protease Dissolve Buffer	2 ml
Buffer AE	10 ml
Protocol	1

Storage conditions and Validity
HiPure FFPE DNA Kits components are guaranteed for at least one year when stored at room temperature.If any precipitates form in the Buffer ATL,warm at 55ºC to dissolve. Proteinase K dry powder is preserved at room temperature. After dissolving, Proteinase K needs to be stored at -20^oC.

Materials and Equipment to be Supplied by User

1. Heat block or water bath capable of 55ºC and 90ºC

2. Xylene or Deparaffinization Buffer (non-toxic)

3. 100% ethanol

4. Add Protease Dissolve Buffer to the Proteinase K, final concentration is 20mg/ml.Store at -20ºC.

5. Dilute Buffer GW1 and Buffer GW2 with 100% ethanol and store at room temperature

Troubleshooting Guide
1. Clogged HiPure DNA Mini Column I

Too much samples: reduce sample amount, paraffin sections do not over 8~10 pieces.
Poor lysis for sample: Tissue sample must be cut or minced into small pieces. Increase incubation time at 56ºC to 3~6 hours to ensure tissue lysed completely.
Insoluble impuri

ties in lysis buffer: if there is still impurity particles in lysis buffer, centrifuge at 10,000 x g for 3 minutes to remove impurities completely.

2. Low or no recovery

Refer to clogged HiPure DNA Mini Column I.
Incomplete crosslinking removal: increase incubation tome at 90ºC to 90~120 minutes, remove protein crosslinking with DNA completey.
Buffer GW1/GW2 did not add with 100% ethanol before use.
Poor elution: elution buffer must be added to the middle of membrane, increase elution volume or times.
Poor Proteinase K activity: prepare new Proteinase K buffer, store at -20ºC.