Description
The kit is used for in vitro qualitative detecting the ORF1ab/N genes of 2019 new virus in respiratory specimens including oropharyngeal swabs, nasopharyngeal swab, sputum and bronchoalveolar lavage fluid, and performing D950N mutation detection of Delta variant of 2019 new virus. As reported in GISAID database, more than 95% Delta variant of 2019 new virus carry D950N mutation. Primer sets and FAM labeled probe are designed for specific detection of ORFlab gene of 2019 new virus, VIC labeled probe for N gene of 2019 new virus, ROX labeled probe for D950N mutation of Delta variant. Human RNase P gene extracted concurrently with the test sample provides an internal control to validate nucleic extraction procedure and reagent integrity. Probe targeting human RNase P gene is labeled with CY5.
The kit is highly compatible with Open ended RT PCR instruments with FAM, HEX/VIC, RED/ROX , and CY5 channels. Uni-medica RT-PCR Kit compatible with popular Molarray MA-6000, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM 5,Hongshi SLAN-96S/96P, Roche Light Cycler 480, Molarray MA-1630Q, AGS8830, AGS4800 and other PCR instruments with FAM, HEX/VIC, RED/ROX , and CY5 channel.
Advantages1.One-tube one-step reaction 2.Hihg sensitivity: LOD is 200copies/ml 3.Short reaction time:52 mins on rapid PCR. 4.The whole process is monitored by internal control 5. Short leading time: 1-3 days
Complaint
