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RN38-EASYspin Plus Plant RNA Rapid Extraction Kit
On the basis of the exclusive EASYspin phenol-free and chloroform RNA rapid extraction technology, the company has developed a successful genomic DNA removal column technology that can effectively remove gDNA residues. The obtained RNA generally does not require DNase digestion, and can be used for reverse transcription PCR, fluorescence quantitative PCR Wait for the experiment. The unique lysate rapidly lyses cells and inactivates cellular RNases, the plant RNA booster PLANTaid helps bind polysaccharides and polyphenols and removes by centrifugation, then the lysis mixture is ethanol-regulated for RNA binding adsorption onto a genomic DNA cleanup column, which simultaneously Residual DNA is removed by adsorption, and then RNA is filtered by selective elution. After the filtered RNA was adjusted to the binding conditions with ethanol, the RNA was selectively adsorbed on the silica matrix membrane in the spin column in the state of high order salt, and then passed through a series of rapid rinsing-centrifugation steps, deproteinizing solution and rinsing solution to remove the cells. Metabolites, proteins and other impurities are removed, and finally, low-salt RNase free H20 is used to elute the pure RNA from the silicon matrix membrane.
Product Features:
1. No toxic phenol, chloroform and other reagents are used at all, and steps such as ethanol precipitation are not required.
2. Simple, single sample operation can generally be completed within 25 minutes, the world's easiest and fastest kit.
3. The unique plant RNA booster can effectively bind polysaccharides and polyphenols to improve the removal effect.
4. The exclusively developed genomic DNA removal column technology can effectively remove gDNA residues. The obtained RNA generally does not require DNase digestion, and can be used for reverse transcription PCR, fluorescence quantitative PCR and other experiments.
5. The adaptability is extremely wide, including cotton, pine needles, holly leaves, grape leaves, and more than 100 domestic and foreign kits that failed to extract samples. For a detailed sample list, please refer to the product introduction on the company's homepage.
6. Multiple column rinsing ensures high purity, the typical ratio of OD260/OD280 is 1.9 to 2.2, and there is basically no DNA residue, which can be used for RT-PCR, Northern-blot and various experiments.
Scientific research achievements
A large number of articles have been published using the EASYspin/EASYspin Plus kit, please contact us to request published articles and universities including Beijing Forestry University, Beijing Agricultural College, China Agricultural University, Fruit Tree Research Institute, Academy of Agriculture and Forestry, Forestry Institute, Chinese Academy of Agricultural Sciences and other universities. A large number of use cases in research institutes.
1. Peach fruit, flower, root and leaf: Isolation, characterisation and phylogenetic analysis of resistance gene analogues in a wild species of peach (Prunus kansuensis). Canadian Journal of Plant Science, 2011, 91(6): 961-970
2. Cherry flowers, leaves, jaws and other parts: Over-expression of the PaAP1 gene from sweet cherry (Prunus avium L.) causes early floweri.Journal of Plant Physiology, 2012,Available online 1 December 2012
3. Onion root, stem, bud, leaf, pistil and other parts: Cloning and Expression Analysis of A Putative B Class MADS-box Gene of AcPI in Onion. Scientia Agricultura Sinica, 2012, 45(23):4759-4769
4. Turnip: Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid Desaturase from Brassica rapa. Journal of Genetics and Genomics Volume 39, Issue 1, January 2012, Pages 47-59
5. Turnip 1: EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA RAPA. Arch. Biol. Sci., Belgrade, 65 (3), 863-876, 2013 DOI:10.2298/ABS1303863H
6. Tomato leaves: Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science Bulletin 2012,28(10):132-135
7. Gardenia: Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural Science Bulletin.2012, 28(27):194-198
8. Tung tree fruit: Cui Qinqin, Han Xiaojiao, Chen Yicun, Zhan Zhiyong, Lin Liyuan, Wang Yangdong. Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding Gene (VfBCCP) from Vernicia fordii. SCIENTIA SILVAE SINICAE. 2012, 48( 8): Available online August
9. Tung tree fruit 1: Selection of Reliable Reference Genes for Gene Expression Studies Using Real-Time PCR in Tung Tree during Seed Development. PLoS ONE, 2012, 7(8): e43084
10. Seaweed: Molecular cloning and expression analysis of ribosomal protein S7 gene from Porphyra haitanensis. JOURNAL OF FISHERIES OF CHINA, 2011, 35(12): 1814-1821
11. Dendrobium: Molecular characterization of a mitogen-activated protein kinase gene DoMPK1 in Dendrobium officinale. Acta Pharmaceutica Sinica, 2012, 47 (12): 1703-1709
12. Dendrobium 1: ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale. Symbiotic Germination Genes in D. officinale. August 2013 | Volume 8 | Issue 8 | e72705
Kit Components:
| Kit composition | save | 50 times |
| Lysate RLT | room temperature | 50ml |
| Lysis buffer CLB (gift) | room temperature | 8ml |
| Lysate RLT Plus | room temperature | 25ml |
| Protein Removal RW1 | room temperature | 40ml |
| Rinse fluid RW | room temperature | 10ml |
| RNase-free H2O | room temperature | 10ml |
| PLANTaid | room temperature | 5ml |
| Genomic DNA Cleanup Columns and Collection Tubes | room temperature | 50 sets |
| RNase-free adsorption column RA and collection tube | room temperature | 50 sets |
Shenzhen Smurf Technology Co., Ltd. is a high-tech enterprise certified by Shenzhen High-Tech Enterprise and Innovative Enterprise. In 2018 received the national high-tech enterprise certificate.
Many of our technologically advanced products are able to make you take less detours and do more with less money, such products are cheap and can solve the problem. To replace these head companies, takara NEB, THERMO, Vazyme, Tiangen, TransGen, Qiagen basically a hit. The price is also much more convenient.
First
All nucleic acid purification . All kinds of RNA extraction, microRNA extraction, gel recovery, plasmids, endotoxin-free plasmids, large amounts, small amounts, ultra-micro amounts, complex
LBOBIO is the first choice in this field in China, which is the closest to the world level. Simply put, we can replace 99% of the products you use with Tiangen's better and cheaper camp. Moreover, we can do the complicated samples that basic Tiangen can't do.
Second
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Thirdly
A variety of PCRmixe, ordinary mix, fast mix, high fidelity mix, fluorescence quantitative mix, this positioning is good quality, low price, some if not the world's first, that is 90% of the world's first level.
Fourth
A variety of reverse transcriptase, high temperature reverse transcriptase technology, including the most advanced one-step genome clearance, two-step genome clearance, full premixed reverse transcriptionmix branch technology, China's leading.
The company is committed to providing life science researchers with simple, easy-to-use, trustworthy molecular biology and biochemistry reagent products and professional technical services. At the same time, in response to the disadvantages of small scale, low standard and poor product stability between batches in the bioreagent industry, the company has introduced venture capital from biopharmaceutical companies as strategic partners to introduce biopharmaceutical-grade scale production equipment, processes and procedures into the field of research reagents and provide research reagents with pharmaceutical-grade quality standards. In the future, the company will base on the frontier field of biotechnology, continuously launch new products with stable quality and reasonable price, and make continuous efforts for the development of life science in China hand in hand with the majority of researchers.
Some of the advantageous products and technology platforms are introduced as follows.
1, nucleic acid purification of various samples, especially difficult special samples such as polysaccharide polyphenol-rich plants, manure, soil and other nucleic acid separation.
2, fluorescence quantitative PCR, reverse transcription fluorescence quantitative PCR assay.
3, large-scale fermenter expression pharmaceutical grade equipment purification of scientific research tools enzymes, including a variety of DNA polymerase, T4 ligase, a variety of mutation modification reverse transcriptase, RNA enzyme inhibitors, etc.
4, PCR product cloning, TA clone ligation.
5,Western Blot Full set of related reagents and services.
6, Transfection grade, industrial grade, pharmaceutical grade large-scale plasmid production.
7, Various proteins, cytokines, enzymes and other expression purification small test, pilot test, amplification.
1. How to pick up the best among these models?
A: Tell us your request on product and almost target price, we recommend you the most suitable model. Selling you the most expensive product is not our goal, selling you the most suitable is!
2. How to place order or pay?
A: You can place order to us directly. We offer you PI with bank information and sales contract, you pay and we ship the product.
3. How to transport the product?
A: We transport the product as your requirement. Depending on the quantity and the machine you order or whether you are urgent or not, we will suggest you suitable transportation way. If you order in batch and are not very urgent, it's best to ship by sea. If you order several items and products are not too heavy, you can choose to ship by air. For small products, courier is used commonly.
4. What kind of packing way for product?
A: Depends on which kind product you order. Most of products are packed with cartons. Some products are packed with wooden boxes. All of the packing way is based on the safety of the machine!
5. What kind of warranty will be offer?
A: For qpcr and thermal cyclers, the warranty is 24 months from the delivery date. And others are 12 months from the delivery date. During the warranty time, if any problem, we offer after-sale servies
6.Lead Times?
Lead times can vary depending on your need and freight availability. At the time of your order, a member of our team will provide you with the most accurate and current lead time.
