Broccoli extract is an active ingredient extracted from cruciferous Brassica broccoli as raw material. It contains various nutrients such as protein, sugar, fat, vitamins and carotene. In addition, it also contains isothiocyanates, flavonoids ( Flavonoids) and volatile oils and other pharmacological components.Broccoli extract can prevent breast cancer, rectal cancer and gastric cancer and other cancers, and also has a strong ability to scavenge DPPH free radicals; ascorbic acid can enhance the liver's detoxification ability and improve the body's immunity. The flavonoids contained have the functions of regulating and preventing high blood pressure and heart disease.
Introduction Originating from Europe, broccoli can be regarded as a high-grade vegetable with complete nutrients. It is rich in protein, carbohydrates, fats and vitamins. It mainly eats green flower buds and stems. It has good flavor and high nutritional value. It is very popular among consumers. In addition,broccoli also contains many antioxidant substances, such as flavonoids and polyphenols, which can effectively prevent lung cancer, intestinal cancer and other diseases, so it is known as the "vegetable crown". Nutrient Content The nutritional composition of broccoli is very comprehensive, the main components are protein, carbohydrates, minerals, fat, dietary fiber, carbohydrates, carotene, vitamin A, vitamin C, vitamin E, calcium, etc., especially the protein content of broccoli is cabbage. 2 times that of flowers, vitamin A is 240 times and 6 times that of cabbage flowers and tomatoes, respectively, and the content of calcium is 2 times that of tomatoes. Anticancer effect An enzyme called carotenoid extracted from broccoli has the effect of increasing the activity of carcinogen detoxification enzymes, thereby preventing the occurrence of cancer. The main anticancer active ingredient in broccoli extract is isothiocyanate, which has high biological activity and can induce apoptosis of human hepatoma cells and gastric adenocarcinoma SGC-7901 cells.
Pharmacological Action Anti-oxidize effect Broccoli extract has a strong ability to scavenge DPPH free radicals, indicating that broccoli contains antioxidant substances that can scavenge hydroxyl radicals, alkyl radicals or peroxy radicals, and can also block the lipid peroxygen chain reaction, and scavenge DPPH free radical capacity was positively correlated with the amount of broccoli extract. The scavenging ability of water-soluble extract of broccoli was stronger than that of fat-soluble extract, indicating that broccoli contains a variety of antioxidants with different polarities. Other role Broccoli is also rich in antacids, so eating more broccoli can not only prevent cancer, but also enhance the detoxification ability of the liver and improve immunity. The high fiber can effectively reduce the absorption of glucose in the stomach, lower blood sugar, and effectively control the condition of diabetes. Broccoli not only has preventive and regulatory effects on heart disease and high blood pressure, but also can prevent eye diseases. For pregnant women, eating more broccoli is very beneficial, because it is rich in folic acid, which plays an important role in the growth and development of the fetus. In addition, eating cauliflower and broccoli can enhance memory.
Extraction process Extraction process of broccoli polysaccharide Broccoli → drying, crushing → degreasing, depigmenting → ultrasonic extraction → suction filtration and slag removal → vacuum concentration → alcohol precipitation → drying → crude polysaccharide Weigh 100g of broccoli and dry it in a drying box to Constant weight, crushed and passed through an 80-mesh sieve. Degrease with petroleum ether at a ratio of 1:3, and use 80% ethanol to remove monosaccharides, oligosaccharides and pigments, and repeat the operation 3 times. After drying at 65°C, We designed different material-to-liquid ratios, extraction times, and ultrasonic power for ultrasonic extraction, and the extract was centrifuged at 4000 r/min for 10 min. Then, concentrated under reduced pressure with a rotary evaporator, dialyzed for 24 hours, added 95% ethanol to the dialysate to make the ethanol concentration 80%, left standing overnight, suction filtered, and washed the solid with absolute ethanol, petroleum ether, and acetone, and dried. After the broccoli refined polysaccharide.
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