-Allow all materials, including specimen and test device, recover to 15-25ºC before running the assay.
-Take out the test card from the foil pouch and place it horizontally.
-Use the capillary dropper to suck specimen and place 1 drop (approx.10μL) of the prepared specimen into sample hole "S" of the test card. Then drop 3 drops (approx. 90μL) of the assay buffer into the sample hole. Start the timer.
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.