Disposable Medical Rapid Detection Monkeypox PCR Test Kit Monkeypox Virus PCR Detection Kit

Min.Order: 1,000
Product origin: Jinan, Shandong, China
Infringement complaint: complaintComplaint
US$ 3 ~ 4

Description
 
Product Description
Product NameMonkeypox Virus Detection Kit (F-PCR)
Detection MethodPCR
Sampling MethodPlasma,Rash Exudate
Packing Specifications1T/box,8T/box,12T/box,24T/box,48T/box,96T/box

Test Procedure
1. Take the nucleic acid of the specimen to be tested and the control substance of Negative and Positive properties, shake and mix well, and centrifuge instantaneously for standby.
2. Thaw the kit at room temperature, shake and mix all components, and centrifuge instantaneously for standby.
3. Calculate the volume of PCR reaction solution and primer probe mixture required according to the number of test specimens n, plus Negative-Positive control (2) and loss (0.5). Take the following table as an example, configure PCR amplification reaction solution in a new EP tube, mix it upside down and centrifuge instantaneously.
 
ComponentPCR Amplification Reaction Solution
PCR reaction solution5*(n+2.5)µL
Primer probe mixture10*(n+2.5)µL
Total volume15*(n+2.5)µL
4. Pack the PCR amplification reaction solution into the PCR reaction tube according to 15 µL/tube, and add 5 µL the samples prepared in step 1, positive control and negative control respectively. The operation shall be carried out on ice, press the pipe cover, mix upside down, and centrifuge at 2000 rpm for 10 seconds.
5. Carefully place the reaction tube to be detected in the PCR amplification detector, set the PCR amplification parameters according to the requirements of the following table, and set the target gene fluorescent reporter group as FAM fluorescence, the internal reference gene fluorescent reporter group as Cy5 fluorescence, and the quenched fluorescent group as NFQ-MGB.
6. Set the reaction procedure of PCR according to the following steps:
Step gatheringNumber of cyclesTemperatureTimeTemperature
1195ºC10 minno
24595ºC15 secno
58ºC60 secyes
7. After the sample is put on the machine, save the file and run the program.
8. ABI 7500 software analysis software threshold value setting: the FAM channel threshold value is set to 5000.
TEST RESULTS

1. The negative quality control FAM/Cy5 channel has no signal, indicating that there is no pollution in the test, and the result analysis can be continued.
2. The Ct value of positive quality control FAM/Cy5 channel ≤ 35 indicates that the experimental system is normal and the result analysis can be continued.
3. All test sample reaction holes shall have Cy5 signal, and the CT value of Cy5 signal shall be ≤ 35. If there is no Cy5 signal or Cy5 Ct> 35, it is necessary to re-extract the sample nucleic acid and repeat the test.
4. Detect the FAM channel of the sample. If there is an S-shaped amplification curve and the Ct value is ≤ 35, the sample is positive for monkeypox virus nucleic acid detection; If there is no amplification curve or Ct value > 35, the sample is negative for monkeypox virus nucleic acid detection.

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Packaging & Shipping

Company Profile

                                            Jinan Babio Biotechnology Co., Ltd.

Founded in 2003, Jinan Babio Biotechnology Co., Ltd. is a professional high-tech enterprise which concentrates on the R&D, manufacturing and selling of in vitro diagnostic reagent. The company was successfully listed on the NEEQ (National Equities Exchange and Quotations) in 2014 (security name: Baibo Biotechnology, stock code: 830774). The company has established a strict quality management system and passed ISO9001:2008 and ISO13485:2003 international quality system certifications. The company has internationally advanced fully automatic production equipment and standard purification workshop of pharmaceutical grade. Baibo has strong biotechnology power, and currently has more than 72 independent intellectual property rights, it undertakes a number of national scientific research projects and has 91 medical device registration certificates approved by CFDA. The company's products mainly involve sampling tube, rapid detection kits, microbial detection reagents, POCT detection reagents, biochemical diagnostic reagents, diagnostic equipment and so on. Babio has been actively involved in the R&D and manufacturing of sampling and testing reagents. Currently, the company's products of transport media, nasal/oral sampling swabs, and the products' quality has been recognized by the medical institutions and laboratories of various countries,  In the future, Babio will be committed to providing better technology and services, and continue to struggle for the human health.


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FAQ

Frequently Asked Questions
1. How do I choose a suitable one?
Dear customer, please tell us your detailed needs via email or online, and we will recommend the right one according to your needs.
2. Are your prices competitive?
Dear customers, we are a manufacturer, and we guarantee to provide you with the best quality and competitive prices.
3. How do I pay?
Dear customer, we accept a variety of payment methods, such as wire transfer, Western Union...
4. When will I receive it after payment?
Dear customer, normal models can be shipped within 5-7 days, please contact us to check the shipping time to your address.
5. How to deliver?
Dear customers, we can ship by express, sea and air.
6. Will it be damaged during transportation?
Dear customers, please don't worry, we do standard export packaging.
7. What should I do if I don't know how to use it?
Dear customers, don't worry, we have a detailed description of use, you can also contact us to provide more service support.

For more tiered pricing based on different order quantity and customized requirements,
please do not hesitate to contact us, we are all looking forward to hearing from you very soon.
 
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