Factory Direct Sales Pipes Buffer Plant Cells Adjust and Stabilize pH Value

Min.Order: 1

Product origin: Ezhou, Hubei, China

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US$ 51.3 ~ 75.5

Description

Packaging Specification : Plastic bottles, 500g/bottle

Basic Information

Product name	Pipes
Abbreviation	PIPES	Molecular formula	C8H18N2O6S2
CAS No	5625-37-6	Molecular weight	302.40
Appearance	White crystalline powder	Purity	>99%
Storage Conditions	Room temperature, light-proof and moisture-proof

Product Introduction

PIPES is an amphoteric buffer with a buffer pH range of 7.2 to 7.4. It is insoluble in water but soluble in aqueous solution. PIPES is included in Good's buffer and is widely used in vaccine production, tissue culture, research on cationic efficacy, and separation techniques for biological substances.

usage method

PIPES is different from other buffering agents, such as BIS-TRIS and BICINE, as it cannot form stable compounds with many metal ions, making it very suitable as a buffering agent in metal ion solutions.

There are two pKa values for PIPES, namely 1pKa and 2.67pKa. 1pKa is 6.76 at ambient temperature of 25 ° C, close to physiological pH, and has a buffer range of 6.1-7.5, which is suitable for cell culture work. 2.67pKa has a buffer range of 1.5-3.5 at 25 ° C.

Application

PIPES can be used to purify tubulin by cellulose phosphate chromatography and recombinant GTP by gel filtration. It can bind proteins ARF1 and ARF2 to crystallize transketolase from Escherichia coli as a buffer solution. However, it is worth noting that PIPES is not suitable for redox systems due to its ability to form free radicals.

Product Advantages

1. The fixation of glutaraldehyde in animal and plant tissue samples can lead to lipid loss with significant morphological changes. When using PIPES buffered glutaraldehyde fixative, lipid loss and artifacts are minimized.

2. If immobilized in oxalate buffered glutaraldehyde for supercellular chemical fixation, it is possible to selectively lose the activity of alkaline phosphatase. When using PIPES as a buffer, the activity retention rate will be significantly improved.

3. The binding of glutaraldehyde and formaldehyde in PIPES buffer can achieve excellent immobilization of fungal spores under fluorescence and electron microscopes.

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!

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