CAS# 1132-61-2 Mops Good′ S Buffer 3-Morpholinopropane Sulfonic Acid

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Product origin: Ezhou, Hubei, China

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US$ 30 ~ 40

Description

CAS# 1132-61-2 MOPS Good's Buffer 3-Morpholinopropane Sulfonic Acid

(English name)3-Morpholinopropane sulfonic acid
(Chinese name)3-Morpholinopropane sulfonic acid
(Abbreviation)MOPS
(CAS#)1132-61-2
(Molecular weight)209.26
(Molecular formula)C7H15NO4S
(Storage condition)room temperature, away from light and moisture
(Molecular structure)
(Application)Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
(Product advantage)The purity is ≥99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES,^[1] and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pK_a of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Applications

MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.^[2] Usage above 20 mM in mammalian cell culture work is not recommended.^[3] MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.^[4]

MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe

MOPS buffer is often used in polyacrylamide gel electrophoresis.

To prepare L of MOPS Buffer (10X) (0.2 M, pH 7):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
MOPS free acid (mw: 209.26 g/mol)	41.86 g	0.2 M
Sodium Acetate (mw: 82.03 g/mol)	4.1 g	0.05 M
Disodium EDTA (mw: 372.24 g/mol)	3.72 g	0.01 M