White High-Purity Powder Reagent Da-64 CAS Number 115871-19-7

Min.Order: 10

Product origin: Ezhou, Hubei, China

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US$ 495

Description

Physicochemical properties of DA64

The chromogen substrate DA64 has more advantages in the detection system of peroxidase. The Molecular formula is C19H23N4NaO3, the molecular weight is 378.39, the full name is N - (carboxymethylaminocarbonyl) -4,4 '- bis (dimethylamino) Diphenylamine sodium, the appearance is white to greenish brown or grayish green, and the crystalline powder to powder. 40mg of DA64 can be dissolved in water at a concentration of over 1 mM per 100g of water.

Basic information

pperance:	White Crystal Powder	Purity:	>99%
Molecular Weight:	380.42	Molecular Formula:	C19H25N4NaO3
Grade:	Analytical Grade	Storage Condition:	Store at 2-10 ºC,away from light and moisture
Transportation Condition:	Store at 2-10 ºC	CAS:	115871-19-7

Advantages of DA64

From the perspective of molecular structure, DA64 is similar to Trinder's reagent or TMB, both of which contain the structure of aniline. After POD catalytic oxidation, it can generate color products with specific absorption wavelengths. Maximum absorption wavelength of DA64 λ Max (color rendering wavelength)=727 nm, ε/ H2O2 (extinction coefficient of an H2O2 molecule)=9 × 104. The wavelength of most chromogenic substrates ranges from 400 to 550, while DA64 is as high as 727 nanometers, much higher than MAOS, known for its long chromogenic wavelength (630nm). It has stronger anti-interference ability for complex samples and higher sensitivity for trace detection.

DA64 quantitative hydrogen peroxide

1. Prepare a chromogenic solution, dissolve 3.78 mg of DA64 and 1 mL of 100 units/mL of peroxidase (POD) in PIPES buffer (0.1mol/L, pH=7, Triton X-100 0.5%), and bring to a constant volume of 100 mL to obtain DA64 100 μ Mol/L solution)

2. Mix 3 mL of the prepared solution with 10 μ Mix the sample solution (H2O2: 0-5 mmol/L) and incubate at 37 ° C for 5 minutes.

3. Use a spectrophotometer to measure the absorbance at 727 nm, and use the absorbance difference of the blank (solution mixed with distilled water to replace the sample solution) as the detection value. Use a separately prepared calibration curve to determine the content in the sample.

Company Introduction

Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!

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