Complaint
| Catalog No. | BS62249 |
|---|---|
| Product Name | MYT1 polyclonal antibody |
| Applications | WB IHC |
| Alternative Name | Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase; PKMYT1; MYT1 |
| Swiss-Prot | Q99640 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Application_all | WB: 1:500~1:1000 IHC: 1:50~1:200 |
| Product | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Purification&Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Storage&Stability | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Specificity | MYT1 polyclonal antibody detects endogenous levels of MYT1 protein. |
| BiowMW | ~ 70, 50 kDa |
| Note | For research use only, not for use in diagnostic procedure. |
| Immunogen | Synthetic peptide, corresponding to Human MYT1. |
Background
Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1.
