Complaint
| Catalog No. | BS64534 |
|---|---|
| Product Name | hnRNP D0 (Phospho-S83) polyclonal antibody |
| Applications | WB IHC IF |
| Alternative Name | Heterogeneous nuclear ribonucleoprotein D0; hnRNP D0; AU-rich element RNA-binding protein 1; AUF1; HNRPD; HNRNPD |
| Swiss-Prot | Q14103 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Application_all | WB: 1:500~1:1000 IHC: 1:50~1:200 |
| Product | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Purification&Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Storage&Stability | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Specificity | hnRNP D0 (Phospho-S83) polyclonal antibody detects endogenous levels of hnRNP D0 protein only when phosphorylated at Ser83. |
| BiowMW | ~ 38 kDa |
| Note | For research use only, not for use in diagnostic procedure. |
| Immunogen | Synthetic phosphopeptide derived from human hnRNP D0 around the phosphorylation site of Serine 83. |
Background
hnRNP D/AUF1 binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3' untranslated regions of many protooncogenes and cytokine mRNAs. It also binds to double and single stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single stranded nonmonotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. It may be involved in translationally coupled mRNA turnover (referenced from swissprot).
