Complaint
| Catalog No. | BS62240 |
|---|---|
| Product Name | Estrogen Receptor alpha polyclonal antibody |
| Applications | WB |
| Alternative Name | Estrogen receptor; ER-alpha; ER; ESR1; ESR; NR3A1 |
| Swiss-Prot | P03372 |
| Host | Rabbit |
| Reactivity | Human,Rat |
| Application_all | WB: 1:500~1:1000 IHC: 1:50~1:200 |
| Product | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Purification&Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Storage&Stability | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Specificity | Estrogen Receptor alpha polyclonal antibody detects endogenous levels of Estrogen Receptor alpha protein. |
| BiowMW | ~ 66 kDa |
| Note | For research use only, not for use in diagnostic procedure. |
| Immunogen | Synthetic peptide, corresponding to Human Estrogen Receptor alpha. |
Background
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics
