Deoxyribonuclease 2 (porcine spleen) CAS: 9025-64-3

Min.Order: 30
Product origin: Nanjing, Jiangsu, China
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US$ 107.66

Description
GroupingEnzyme
KeywordsDNase II 
BrandNJDULY
Model E0047
Place of OriginNanjing, China (Mainland)
PaymentT/T
PortShanghai
Supply Capacity100 per piece/Month
Packing30KU/piece
Other packagingplease consult customer service
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Product Description:

CAS: 9025-64-3. 
Grade: BR. 
Vitality: > 1000u/mg. 
Activity is defined as: One Kunitz unit will produce a Δ A260 of 0.001 per min per mL at pH 4.6 at 25 ºC; [Mg2+] = 0.83mM. 
Drying weight loss: < 5.0%. 
Burning residue: < 1.0%. 
Character: White or quasi-white freeze-dried powder. 
It is a double-stranded specific endonuclease, which can degrade double-stranded DNA into oligodeoxynucleotides with 5-phosphate and 3'-OH terminal. 
Single-stranded DNA can also be degraded in the presence of univalent manganese ions. 
Soluble in water, slightly soluble in 30% ethanol . 
Enzyme reaction: deoxyribonucleic acid dinucleotide 5 'phosphate + oligonucleotide 5' phosphate is the most suitable pH 7.8. 
Absorptivity (E1% 280) 11.1. 
The activator is divalent metal ion, and the maximum activation can be obtained from magnesium (Mg2+) + calcium (Ca2+). 
Inhibitors include EDTA and sodium dodecyl sulfate. 
5mmol/L calcium (Ca2+) can be added. 
Purpose: biochemical research. 
Used in molecular biology research. 
Various enzymes that degrade DNA. 
These enzymes hydrolyze the phosphodiester bond on the sugar-phosphate main chain. 
There are two kinds of enzymes: exonuclease and endonuclease. 
There are many kinds of DNAase, some are single-strand specific, some are double-stranded specific, or sequence-specific (such as restriction endonuclease). 
Restriction endonucleases have the ability to recognize specific base sequences, which has been used in gene manipulation techniques to construct artificial clones. 
This product is for scientific research only. 
Storage:-20 ºC

Company Information

Nanjing Duly Biotech Co.,Ltd 

has been established for 11 years. It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.

 

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