His-Tag Polyclonal Antibody

Min.Order: 100

Product origin: Nanjing, Jiangsu, China

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US$ 275

Description

Product Name	His-tag polyclonal antibody
Catalog No.	AP0032
Swiss-Prot	N/A
Host	Rabbit
Reactivity	All
Applications	WB IP IF
Application_all	\"WB: 1:2000~1:5000 IP: 1:50~1:200 IF: 1:50~1:200\\\"
BiowMW	N/A
Alternative Name	6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Purification&Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Conjugate	Unconjugated
Modification	Unmodification

Western blot (WB) analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:2000 (lane 1) and 1:5000 (lane 2). Each lane was loaded with 10 μg of E. coli lysate.
\"Western blot (WB) analysis of His-tag polyclonal antibody at 1:5000 dillution Lane1:HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His.\"
\"Western blot (WB) analysis of His-tag polyclonal antibody at 1:5000 dillution Lane1:HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His.\"

\"Western blot (WB) analysis of His-tag polyclonal antibody at 1:5000 dillution Lane1:HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His.\"

Background :
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.

Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :
His-tag polyclonal antibody detects over-expressed or recombinant proteins containing the 6× His epitope tag

Immunogen :
Synthetic peptide His-tag.

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