Biochemical Laboratory Reagent Enzyme Preparation Homocysteine Methyltransferase, Hmt

Min.Order: 10

Product origin: Ezhou, Hubei, China

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US$ -1

Description

Basic information

Dosage form:	Colorless clear liquid or freeze-dried powder	Molecular weight:	51kDa(SDS-PAGE detection)
ph stability:	pH6.5-8.5	Purity	≥ 90% (SDS-PAGE detection)
Enzyme activity:	70 U/mg	Transportation Conditions:	Low temperature, ice bag
Source:	Gene recombination expression	Safety tips:	Not applicable for human experiments
Resolution:	It can be re dissolved in pure water containing 30% glycerol and stored at 4 ºC for one year; Divide according to the dosage and store at -20 ºC for two years, avoiding repeated freeze-thaw as much as possible.
Definition of enzyme activity:	Under specific reaction conditions at 37 ºC and pH 8.3, the catalytic reaction results in a 0.01 decrease in the absorbance of NADH at 340nm per minute, defined as an enzyme activity unit.

Product Introduction

Homocysteine (Hcy) is a sulfhydryl amino acid and a new independent and important risk factor for cardiovascular disease. At present, Hcy has been used as a detection indicator for cardiovascular diseases both domestically and internationally. Among the Hcy detection methods, the Hcy detection kit is the fastest, most sensitive, suitable for large-scale sample detection, and can be automatically analyzed on a biochemical analyzer. The kit uses a cyclic enzyme method, and one of the most core enzymes in the kit is homocysteine methyltransferase (HMT).

The relationship between enzyme preparations, new Trinder's reagents, and Good's buffer

There are many principles of in vitro diagnostic kits, and the most common one is a diagnostic kit based on the Trinder reaction principle (also known as enzyme method), which can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglycerides, etc. in blood tests. This diagnostic kit typically includes important components such as enzymes, new Trinder's reagents, and Good's buffer.

By adding the new Trinder's reagent to the test substance, a color reaction occurs, and the value of the test item is calculated through absorbance measurement. For example, the values of blood sugar and uric acid in serum. The new Trinder's reagent has the advantages of high water solubility, UV absorption of color reaction products>540nm, wide pH range requirements for color reactions, and high sensitivity for color reactions.

The use of enzyme preparations in the Trinder reaction is due to the presence of too many substances in the serum, and the specificity of enzyme preparations. For example, adding glucose oxidase will only catalyze the oxidation of blood sugar to generate hydrogen peroxide, without catalyzing the oxidation of uric acid and other substances present in the serum, ensuring the accuracy of the detection results as much as possible. However, pH value has a significant impact on enzyme activity, and each enzyme has its own high activity pH range. At this point, Good's buffer is needed to adjust the pH value.

Different Good's buffers can provide suitable buffer intervals for different enzymes. Good's buffer has the advantages of high water solubility, bottom cell membrane permeability, stable acid-base dissociation constant, low metal chelating ability, high chemical stability, etc. In summary, enzyme preparations, new Trinder's reagents, and Good's buffer complement each other in the diagnostic kit, making corresponding contributions to the precise detection of diseases.

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!

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