Rna Protein Electrophoresis Buffer Preparation 10 Mops Buffer

Product Introduction

MOPS is 3-morpholinopropane sulfonic acid, CAS number 1132-61-2, sulfonic acid substance, is a common biological buffer, often used to prepare RNA and protein electrophoresis buffer.

This product is mainly composed of MOPS, sodium acetate, and EDTA. It is prepared with nuclease-free water and filtered by a microporous membrane. It is suitable for RNA formaldhyde deformed electrophoresis. After testing, this product has no RNase activity.

This product contains 0.4 M MOPS, 0.1 M sodium acetate, 10 mM EDTA, pH 7.0±0.1.


Storage and transportation

Transport at room temperature, store at room temperature, avoid direct sunlight, valid for 24 months.


Instructions for use

This product is a ten-fold concentrated solution, which needs to be diluted 10 times before use, that is, every 10 mL of 10×MOPS Buffer is mixed with 90 mL of nuclease-free water (G4700 or G3004 is recommended) before use.

When preparing an agarose gel for RNA formaldhyde denaturation electrophoresis, you can operate as follows: Take the preparation of 1% agarose gel as an example, weigh 0.5 g agarose and add 36 mL RNase-Free Water (G4700 or G3004 is recommended) ), after heating and melting, add 5 mL 10×MOPS Buffer (G3373 is recommended). When the solution is cooled to not hot, add 9 mL of 37% formaldhyde solution, add nucleic acid dye EB as needed, mix and pour the gel.


Precautions

1. Pay attention to the operation when using, use a sterile non-enzyme tip to absorb the solution to avoid RNase contamination.

2. This product tends to turn yellow when exposed to light. It is recommended to store in a cool and dark place. When the solution is light yellow, it will not affect the use. If the color is too dark, it is recommended to stop using it.

3. For your safety and health, please wear lab coats and disposable gloves for operation.